Organelle Level Features

Common Features

These features are calculated for all organelles

• label: number index of each individual organelle

• area: the area of the organelle in \({\mu}\)m²

• perimeter: the perimeter of the organelle in \({\mu}\)m

• centroid-0: the y coordinate of the center of the organelle, measured from the top left corner of the image

• centroid-1: the x coordinate of the center of the organelle, measured from the top left corner of the image

• axis major length: the length of the major axis of the ellipse that has the same normalized second central moments as the organelle

• axis minor length: the length of the minor axis of the ellipse that has the same normalized second central moments as the organelle

• cell id: the index of the cell that the organelle is located in cell id of 0 corresponds to the sinusoid

• aspect ratio: ratio of the major axis to the minor axis

• boundry distance: distance from the centroid of the organelle to the nearest boundary of the cell in pixels

• ascini_position: relative position of the centroid of an organelle on the ascinus 1 = portal vein and -1 = central vein

Mitochondria Specific Features

• ** solidity**: ratio of pixels in the organelle to the pixels in its convex hull

• aspect type 1: mitochondria with an aspect ratio < 1.2

• aspect type 2: mitochondria with an aspect ratio between 1.2 and 2

• aspect type 3: mitochondria with an aspect ratio > 2

Lipid Droplet Specific Features

• area type 1: lipid droplets with an area less than 2.41 \({\mu}\)m²

• area type 2: lipid droplets with an area between 2.41 and 9.64 \({\mu}\)m²

• area type 3: lipid droplets with an area greater than 9.64 \({\mu}\)m²

Cell Level Features

• area: the area of the cell in \({\mu}\)m²

• centroid-0: the y coordinate of the center of the cell, measured from the top left corner of the image

• centroid-1: the x coordinate of the center of the cell, measured from the top left corner of the iamge

• label: number index of the specific cell, used to match to the cell id* feature of specific organelles

• density: the number of organelles per \({\mu}\)m²

• average area: mean area of all organelles of a specific type within a cell (\({\mu}\)m²

• percent total area: ratio of the total area occupied by organelles of a specific type to the total area of the cell

• distance from edge: ratio of the distance to the center of of the cell to the sum of the distance to the center of the cell and the distance to the closest edge

• aspect ratio: mean aspect ratio of all organelles of a specific type within a cell

• solidity: mean solidity of all organelles of a specific type within a cell

• percent type: percent of all organelles within a cell that are a specific type, see aspect type for mitochondria and area type for lipid droplets

• ascini position: relative position of the centroid of the cell on the ascinus 1 = portal vein, -1 = central vein